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Table 1 Summary metrics derived from complete reference sample sets: five samples, three replicates each, and three rounds

From: Summarizing performance for genome scale measurement of miRNA: reference samples and metrics

Measurement   Data Source miRNA Detected Log2 Signals AUCc Deviation
Process Technology by Round   Non- TrueNeg TruePos Lower Upper Range All Within Log2 Ratio Proportion
   Pure Mixes Reps Selective 1-to-1a Brain Placenta Limitb Limit Range Median IQR Sum
A NGS 3 3 3 928 56 103 104 5.8 20.0 14.3 0.959 0.996 -0.013 0.303 0.375
4 4 3 935 72 101 105 5.7 20.2 14.5 0.977 0.990 0.007 0.309 0.384
5 5 3 859 59 107 105 6.3 20.3 13.9 0.909 0.963 -0.053 0.373 0.355
B NGS 3 3 3 868 56 134 47 7.3 20.2 12.8 0.905 0.953 -0.085 0.387 0.508
4 4 3 721 38 102 58 5.7 19.0 13.2 0.922 0.951 -0.078 0.328 0.388
5 5 3 641 43 78 44 5.8 19.2 13.4 0.906 0.928 -0.052 0.322 0.389
C NGS 3 3 3 421 35 29 49 5.3 16.3 11.0 0.944 0.971 0.030 0.266 0.332
4 4 3 277 22 16 30 6.3 11.8 5.6 0.831 0.829 0.088 0.557 0.359
5 5 3 433 29 24 31 10.9 12.4 1.5 0.853 NDd -0.104 0.875 0.480
D NGS 3 3 3 248 16 18 9 8.6 10.6 2.0 0.781 NDd -0.124 0.627 0.803
4 4 3 290 13 13 2 6.6 10.6 4.0 0.956 NDd -0.134 0.534 0.402
E Digital Hybridization 3 3 3 693 65 8 24 7.1 13.3 6.1 0.947 1.000 -0.075 0.392 0.460
4 4 3 633 17 8 23 5.6 14.4 8.8 0.985 0.997 -0.095 0.379 0.304
5 5 3 586 52 16 37 13.9 15.3 1.4 0.882 1.000 -0.238 0.599 0.473
  1. aLiver-selective miRNA or miRNA with approximately equal signals in both brain and placenta
  2. bLower limits for NGS and digital hybridization were calculated with at 95% of values falling within ± 0.585 log2 difference from mean
  3. cROC curves were prepared with P-values using three replicates of Mix1 and Mix2
  4. dNot determined (ND) due to insufficient number of true negative or true positive values