Table 3 Summary of variable genes across M. synoviae strains MS-H, MS53 and WVU-1853

N-acetylneuraminate lyase variant

MSH_00300

–

–

Transcriptional regulator

MSH_00310^b

MSH_00330^b

MS53_0024

VY93_00145

Translation elongation factor G

MSH_00500

MS53_0039^b

VY93_00220^b

Multiple sugar ABC transporter, ATP-binding protein

MSH_01200^c

MS53_0102

VY93_00560

Hypothetical protein

MSH_01840^c

–

VY93_00865

Hypothetical protein

MSH_01960

–

VY93_00910

Hypothetical protein

MSH_01930

MSH_01970

–

VY93_00915

Type II restriction enzyme MjaIII

MSH_02140

–

VY93_01010

VY93_02580

Methyl-directed repair DNA adenine methylase

MSH_02150

–

VY93_01015

VY93_02585

3′-to-5′ oligoribonuclease A

MSH_02940^b

MSH_02950^b

MSH_02960^b

MS53_0278^b

MS53_0279^b

VY93_01505^b

VY93_01510^b

Aspartate-ammonia ligase

MSH_02990

MS53_0281^b

VY93_01515

Hypothetical protein

MSH_03480^b

MSH_03490^b

MS53_0293

VY93_01595^b

Asparaginyl-tRNA synthetase-related protein

MSH_03500

MS53_0291^b

MS53_0700^b

VY93_01590^b

Deoxyribose-phosphate aldolase

MSH_03520

–

–

Oligopeptide transport ATP-binding protein OppF

MSH_03740^b

MSH_03750^b

MS53_0345

VY93_01875

Lipoprotein

MSH_03790

MS53_0349

VY93_01895^b

VY93_01900^b

Hypothetical protein

MSH_04620

MS53_0430^b

VY93_02305

Hypothetical protein

MSH_04790^b

MS53_0442

Hypothetical protein

MSH_04880

VY93_02420

Hypothetical protein

MSH_04960

MS53_0458^b

MS53_0459^b

VY93_02465

Hypothetical protein

MSH_05850^b

MSH_05860^b

MS53_0713^b

MS53_0540^b

VY93_02935^b

CRISPR-associated protein, Csn1

MSH_06430^b

MSH_06440^b

MS53_0582

VY93_03200^b

VY93_03205^b

IS30 family transposase

MSH_07180

MSH_07210

–

VY93_00200

VY93_00900

VY93_01540

VY93_02375

ATP-dependent DNA helicase

MSH_07190

–

–

Hypothetical protein

MSH_07360^b

MS53_0668

VY93_03635

Hypothetical protein

MSH_07370^b

MS53_0669^b

VY93_03640

ABC transporter ATP-binding and permease protein

MSH_07380

MS53_0670^b

VY93_03645^b

Hypothetical protein

MSH_07610^c

MS53_0680

VY93_03760^b

PTS system enzyme IIB component

MSH_07640^b

MS53_0682

VY93_03770

Ascorbate-specific PTS system, EIIC component

MSH_07650^b

MSH_07660^b

MSH_07670^b

MSH_07680^b

MS53_0683

VY93_03775^b

Type III restriction-modification system methylation subunit

MSH_07490^b

MSH_07500^b

–

VY93_03700^b

VY93_03705^b

Hypothetical protein

MSH_07510

–

VY93_03710^b

Hypothetical protein

MSH_07520

–

VY93_03720^b

Hypothetical protein

MSH_07530

–

VY93_03725^b

Hypothetical protein

MSH_07540 ^b

–

VY93_03730

Integrase

MSH_07560

–

VY93_03735

Type I restriction-modification system, specificity subunit S

MSH_07570^b

MSH_07580^b

–

VY93_03740^b

VY93_03745^b

Type I restriction-modification system, restriction subunit R

MSH_07590

–

VY93_03750

Type I restriction-modification system, DNA-methyltransferase subunit M

MSH_07600

–

VY93_03755