Fig. 1

Amino-acid sequence alignment of StPTB1/6-like orthologues in select storage root crops. Gray boxed letters represent the residues in PTB1/6-like orthologues of storage root crops identical to StPTB1 and StPTB6, letters highlighted in red represent the residues identical in at least two PTB1/6-like orthologues, whereas residues not highlighted represent non-conserved residues among these PTB1/6-like orthologues. Four RNA recognition motifs (RRMs) are underlined in red. Potential canonical RNPs in each RRM are highlighted in yellow. Clustal consensus sequences are represented by asterisks below the alignment. The amino-acid sequences of PTB1/6-like orthologues in storage root crops are aligned to StPTB1 and StPTB6 amino-acid sequences in potato as a reference. CmRBP50 RRM and RNP sequences were used for identifying potential RRM and RNPs in these StPTB1/6-like orthologues [5]. Among the different PTB1/6-like variants identified in each storage root crop (Table 1), one protein per crop with the best coverage and identity were considered for the sequence alignment shown here. These accessions for protein sequences were: ItPTB1/6-like (itf09g10450.t1), MePTB1/6-like (Manes.18G093400.1), DcPTB1/6-like (XP_017247842.1), RsPTB1/6-like (XP_018451916.1) and BvPTB1/6-like (XP_010681101.1). PTB, polypyrimidine tract-binding; St, Solanum tuberosum; It, Ipomoea trifida; Me, Manihot esculenta; Bv, Beta vulgaris; Dc, Daucus carota; Rs, Raphanus sativus