Fig. 4

JAK/STAT3 Controls Proper Imprinting of the Dlk1-Dio3 Region and the Expression of Key Pluripotent Genes. a Expression fold change from RNA-seq analysis for significantly downregulated lincRNAs in Jaki vs. DMSO for S2 reprogrammed cells. Genes exclusively detected in DMSO but not Jaki-condition were also shown. b qPCR analysis of Gtl2-Rian-Mirg gene expressions in two different reprogramming conditions and stages. R1-ESC was used as the control. Bars represent mean ± SD from three independent biological repeats. *: p < 0.05, **: p < 0.01. c Expression fold change from RNA-seq analysis for pluripotent genes significantly downregulated between Jaki- vs. Ctl-treatment at S2. Genes detected exclusively in Ctl but not in Jaki-treatment were also shown. Socs3 expression was shown as an indictor for inhibited STAT3 activity. d qPCR analysis of key pluripotent gene expression under two different reprogramming conditions and stages. R1-ESC was used as the control. Bars represent mean ± SD from three independent biological repeats. eOct4, eSox2: endogenous Oct4 and Sox2. Arrowhead: expression not detected. **: p < 0.01. e Venn Diagram depicting the relationship among the pluripotent genes significantly downregulated between Jaki- vs. Ctl-treatment at S2, the STAT3 direct targets, and the makers specific for either naïve-state pluripotent ESCs or primed-state EpiSCs