Fig. 2

Relationship between polymerase pausing and chromatin accessibility. a Scatterplot of Start-seq (x-axis) vs. nuclear RNA-seq (y-axis) signal. b Pause index (Start-seq/RNA-seq) for obsTSSs from different classes of genes, including immune response and transcription factor genes (more paused than average). c ATAC-seq signal and predicted nucleosome occupancy at obsTSSs stratified by pausing index (PI). High-PI obsTSSs have higher chromatin accessibility at the minus-1 nucleosome free region, despite also having higher predicted nucleosome occupancy in that region