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Fig. 3 | BMC Genomics

Fig. 3

From: High fidelity hypothermic preservation of primary tissues in organ transplant preservative for single cell transcriptome analysis

Fig. 3

Cell types in kidney resident immune cells and the impact of preservation on cell type heterogeneity. a Definition of putative cell clusters on 2d tSNE. b Distribution of putative clusters in fresh and preserved tissues (6 h-4d). c Identification of putative clusters with known cell types using hierarchical clustering on a canonical panel of genes defining major immune lineages. d Expression of representative genes differentially expressed in cluster 8 (color bar shown in log2(rpm + 1)). e Hierarchy of enriched ontology terms for differentially expressed genes in putative cluster 8. (All coloring and numbering for cluster ID in (b), (c) follow those in (a); coloring for preservation time follows the legend in Fig. 2b)

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