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Table 3 Relative expression of the set of isoforms of four loci (TIMP1, ITGA5, ANXA2 and LITAF) in HIGH (N = 26) vs LOW (N = 26) pigsa

From: Differential expression of mRNA isoforms in the skeletal muscle of pigs with distinct growth and fatness profiles

Feature ID

Feature transcript ID

Transcript ID

Length (bp)

Typeb

Relative expression mean (%)

CLC Bio

STAR/RSEM/DESeq2

Log2

(FC)

P-value

q-value

Log2

(FC)

P-value

q-valuec

ENSSSCG00000004578

ENSSSCT00000034155

ANXA2–201

1609

Protein coding

0.64

0.21

6.77E-01

1.00E + 00

0.07

9.20E-01

NA

ENSSSCG00000004578

ENSSSCT00000005057

ANXA2–202

1455

Protein coding

99.36

0.70

3.32E-06

7.23E-03

0.69

4.56E-06

1.58E-03

ENSSSCG00000000293

ENSSSCT00000000314

ITGA5–201

4445

Protein coding

99.00

0.85

2.64E-05

2.82E-02

0.85

2.54E-06

1.21E-03

ENSSSCG00000000293

ENSSSCT00000035821

ITGA5–203

1255

NMD

0.34

−0.03

9.18E-01

1.00E + 00

ENSSSCG00000000293

ENSSSCT00000034427

ITGA5–204

1013

NMD

0.71

−0.08

1.00E + 00

1.00E + 00

ENSSSCG00000000293

ENSSSCT00000033141

ITGA5–205

766

NMD

0.48

−0.29

1.00E + 00

1.00E + 00

−0.01

9.97E-01

NA

ENSSSCG00000012277

ENSSSCT00000013426

TIMP1–001

931

Protein coding

97.86

2.57

4.61E-07

1.85E-03

0.79

2.01E-04

1.32E-02

ENSSSCG00000012277

ENSSSCT00000033796

TIMP1–002

598

Protein coding

0.38

1.12

5.56E-01

1.00E + 00

0.38

8.98E-01

NA

ENSSSCG00000012277

ENSSSCT00000034602

TIMP1–003

641

Protein coding

1.57

1.19

8.47E-01

1.00E + 00

−0.19

7.39E − 01

NA

ENSSSCG00000012277

ENSSSCT00000036308

TIMP1–004

173

Protein coding

0.19

3.05

4.21E-01

1.00E + 00

0.18

9.52E-01

NA

ENSSSCG00000024982

ENSSSCT00000036552

LITAF-201

2190

Protein coding

88.45

1.01

1.02E-04

4.82E-02

0.79

1.21E-04

1.04E-02

ENSSSCG00000024982

ENSSSCT00000025103

LITAF-202

2370

Protein coding

11.55

-0.04

1.00E + 00

1.00E + 00

0.49

7.52E-01

NA

  1. aDifferentially expressed mRNA isoforms that remained significant after correction for multiple testing (q-value < 0.05 and ±0.6 log2Fold-Change) are shown in bold. A positive log2FC means that the gene is upregulated in HIGH pigs
  2. bNMD Nonsense-mediated mRNA decay
  3. cFor multiple testing correction, DESeq2 carries out a filtering step based on the average expression strength of each gene across all samples with the aim of discarding genes which are likely to loose significance after correcting for multiple testing. The purpose of this filtering step is to increase statistical power by reducing the list of candidate genes to be tested. The q-values of the genes which do not pass the filtering step are set to NA