Fig. 2

Heatmap showing hierarchical clustering of 532 differentially expressed genes among treatments. The log2 ratio values of DEG abundance were used for hierarchical cluster analysis with the R pheatmap package. Red and blue indicate relative over- or under-expression of genes, respectively. Dietary treatments were corn silage-based diets that consisted of 20%, 40%, 60%, and 80% corn silage (on a DM basis, namely the S20, S40, S60, and S80 groups)