Fig. 6

Regulation of hepatic cholesterol biosynthesis in different forage-to-concentrate ratios fed to heifers. Major metabolic intermediates are shown in red font and genes are shown in black font. Line charts represent the significant differentially expressed genes associated with cholesterol biosynthesis in this study, and mean values of FPKM are displayed. S20, 20% forage in diets; S40, 40% forage in diets; S60, 60% forage in diets; S80, 80% forage in diets. HMGCS1, 3-hydroxy-3-methylglutaryl-CoA; HMGCR, 3-hydroxy-3-methylglutaryl-CoA reductase; MSMO1, methylsterol monooxygenase 1; MVK, mevalonate kinase; MVD, mevalonate diphosphate decarboxylase; IDI1, isopentenyl-diphosphate delta isomerase 1; FDPS, farnesyl diphosphate synthase; LSS, lanosterol synthase; DHCR7, 7-dehydrocholesterol reductase; FPKM, fragments per kilobase of exon per million fragments mapped