Fig. 4

Relative cleavage site position within genes and reading frame dependent cleavage bias. a The relative distance that cleavage sites fall within protein coding genes was plotted in a histogram (see materials and methods). Cleavage sites are shown in turquoise, 5′ monophosphate sites occurring at the location of a TSS (i.e. potential 5′ pyrophosphatase sites) are shown in yellow. The remaining sites are distributed relatively evenly throughout the ORF. b The reading frames of cleavage products were determined for both positive (navy blue) and negative (red) stranded transcripts. Cleavage at site 1 occurs 5′ to nucleotide 1 (↓N1 N2 N3), cleavage at site 2 occurs 5′ to nucleotide 2 (5’-N1↓N2 N3) and cleavage at site 3 occurs 5′ to nucleotide 3 (5’-N1N2↓N3)