Fig. 2

Analysis of RNA structural binding preferences for a large compendium of RBPs. a) Distribution of paired to unpaired binding preference log ratios for 205 RBPs shows almost all proteins in this dataset prefer to bind unpaired regions. b) Distribution of loop to external binding preference log ratios for 205 RBPs reveals more proteins in this dataset prefer to bind loop regions, and many may bind both. c) RNA structural binding preference improves in vitro binding prediction. Correlation results over 488 paired experiments uncovers that RNA structure plays a significant role in protein-RNA interactions. d) RNA structural binding preference improves in vivo binding prediction. AUC results of 96 paired eCLIP and RNAcompete experiments over 21 joint proteins demonstrate that RNA structural binding preferences learned from in vitro data correlate well with protein-RNA interactions measured in vivo