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Table 4 PMC CRMs Validated by Reporter Gene Assays

From: Global analysis of primary mesenchyme cell cis-regulatory modules by chromatin accessibility profiling

Putative CRM Tested

Total No. Injected Embryos

No. GFP-Positive Embryos

No. Embryos Expressing GFP in PMCs

Sp-kirrelL (WHL22.699052)

288

88 (31%)

88

Sp-mitf (WHL22.677144)

230

170 (74%)

170

Sp-msp130r2 (WHL22.451280)

244

106 (43%)

106

Sp-sh2d5 (WHL22.637506)

80

43 (54%)

43

SPU_023052 (WHL22.364101)

149

28 (19%)

28

Novel PMC DE Gene (WHL22.691495)

160

109 (68%)

109

Intergenic

78

35 (45%)

35

Sp-hypp_2386 (WHL22.239326)

86

53 (62%)

53

Sp-c-lectin/PMC1 (WHL22.411805)

257

27 (11%)

27

  1. Nine out of 31 injected reporter constructs showed PMC-specific GFP expression at 48 hpf. No ectopic expression was observed