Fig. 1From: Analytical parameters and validation of homopolymer detection in a pyrosequencing-based next generation sequencing systemExperimental setup. In the plasmid system 4-to 6-mer homopolymers (HPs) were generated and tested using different primer sets. In the CFTR assay, 17 samples with known genotype were tested. This test system was divided into three parts, exons containing no HPs, HP-containing exons and the 2184 insA mutationBack to article page