Fig. 5From: Slow darkening of pinto bean seed coat is associated with significant metabolite and transcript differences related to proanthocyanidin biosynthesisExpression analysis of highly DE genes in RD and SD pinto lines. a Four different stages (30, 50, 150 and 350 mg seed weight) of CDC Pintium seeds during development. b Total RNA extracted from seed coat was collected from CDC Pintium (RD) and 1533–15 (SD) pinto seeds at the stages shown in A and was used for qRT-PCR analysis using gene-specific primers. Relative expression corresponds to mean value in two biological replicates and 3 technical replicates per biological replicate. Error bar indicates SEMBack to article page