Fig. 6From: Slow darkening of pinto bean seed coat is associated with significant metabolite and transcript differences related to proanthocyanidin biosynthesisExpression analysis of DE genes located on chromosome 7 in RD and SD pinto lines. a A schematic diagram of chromosome 7 in pinto bean showing the location of SSR markers and Sd gene. b Gene expression analysis of DE genes located on chromosome 7. Total RNA extracted from seed coat was collected from CDC Pintium (RD) and 1533–15 (SD) pinto seeds at 4 different stages of development and was used for qRT-PCR analysis using gene-specific primers. Relative expression corresponds to mean value in two biological replicates and 3 technical replicates per biological replicate. Error bars indicate SEMBack to article page