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Fig. 6 | BMC Genomics

Fig. 6

From: Slow darkening of pinto bean seed coat is associated with significant metabolite and transcript differences related to proanthocyanidin biosynthesis

Fig. 6

Expression analysis of DE genes located on chromosome 7 in RD and SD pinto lines. a A schematic diagram of chromosome 7 in pinto bean showing the location of SSR markers and Sd gene. b Gene expression analysis of DE genes located on chromosome 7. Total RNA extracted from seed coat was collected from CDC Pintium (RD) and 1533–15 (SD) pinto seeds at 4 different stages of development and was used for qRT-PCR analysis using gene-specific primers. Relative expression corresponds to mean value in two biological replicates and 3 technical replicates per biological replicate. Error bars indicate SEM

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