Table 2 Transcriptome assembly and annotation statistics. To avoid confusion between Pomacea and Pila, the latter taxon is not abbreviated as “P.”
From: AmpuBase: a transcriptome database for eight species of apple snails (Gastropoda: Ampullariidae)
Items | L. nyassanus | Pila ampullacea | A. platae | M. cornuarietis | P. diffusa | P. scalaris | P. canaliculata | P. maculata |
|---|---|---|---|---|---|---|---|---|
De novo assembly | ||||||||
Assembled bases | 164,160,894 | 238,879,002 | 214,102,711 | 159,734,791 | 168,090,829 | 141,684,727 | 536,808,768 | 145,979,415 |
Assembled transcripts | 152,931 | 277,864 | 203,935 | 187,959 | 204,576 | 126,582 | 499,932 | 200,397 |
Assembled unigenes | 122,779 | 212,935 | 156,912 | 161,143 | 171,676 | 98,100 | 215,456 | 154,712 |
Clustered transcripts | 129,455 | 221,653 | 165,023 | 161,069 | 173,606 | 105,046 | 355,408 | 154,700 |
Clustered unigenes | 114,869 | 192,301 | 142,773 | 147,375 | 157,064 | 89,910 | 211,621 | 136,742 |
Unigenes (transcripts) | 22,204 (29,317) | 35,828 (46,232) | 20,730 (28,927) | 29,400 (35,994) | 28,408 (36,112) | 20,829 (28,847) | 28,755 (57,048) | 28,782 (35,063) |
Unigene N50 (bp) | 1740 | 1683 | 1803 | 1440 | 1485 | 1629 | 1509 | 1320 |
Unigene length (bp) - average (min - max) | 1222 (300–31,476) | 1182 (300–19,023) | 1281 (300–15,984) | 1054 (300–23,508) | 1076 (300–25,756) | 1163 (300–13,624) | 1074 (300–40,192) | 974 (300–17,707) |
BUSCO | ||||||||
Complete (%) | 86.83 | 92.41 | 82.09 | 77.82 | 79.95 | 80.43 | 80.07 | 77.46 |
Fragmented (%) | 4.15 | 3.68 | 4.74 | 13.52 | 11.63 | 8.78 | 7.47 | 12.81 |
Annotation (unigenes) | ||||||||
NCBI nr | 17,065 (76.86%) | 27,254 (76.07%) | 16,051 (77.43%) | 22,579 (76.80%) | 21,405 (75.35%) | 16,705 (80.20%) | 20,051 (69.73%) | 21,625 (75.13%) |
GO | 10,697 (48.18%) | 18,717 (52.24%) | 9852 (47.53%) | 14,274 (48.55%) | 13,519 (47.59%) | 10,394 (49.90%) | 12,216 (42.48%) | 13,671 (47.50%) |
KEGG | 3783 (17.04%) | 5467 (15.26%) | 3546 (17.11%) | 4215 (14.34%) | 4061 (14.30%) | 3801 (18.25%) | 3693 (12.84%) | 4059 (14.10%) |