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Fig. 5 | BMC Genomics

Fig. 5

From: The deregulated microRNAome contributes to the cellular response to aneuploidy

Fig. 5

hsa-miR-10a-5p levels and activity are increased in aneuploid cel lines. a hsa-miR-10a-5p expression analysed by quantitative real-time PCR in 18 different aneuploid model cell lines. Boxplots present minimal, maximal and the mean value. Relative expression levels normalized to corresponding parental cell line is shown. b Luciferase reporter construct to determine hsa-miR-10a-5p endogenous gene repression activity. Hsa-miR-10a-5p targets its binding site in a synthetic 3’UTR of the Renilla luciferase mRNA resulting in translational repression and/or degradation of Renilla luciferase mRNA. Renilla luciferase signal is normalized to the internal Firefly luciferase control (see Methods). c Endogenous hsa-miR-10a-5p activity determined by psiCheck2-10a luciferase reporter assay with hsa-miR-10a-5p binding site in synthetic 3’UTR. HCT116-derived and RPE1-derived cell lines were transfected with psiCheck2-10a luciferase reporter construct. Luciferase reporter assay was conducted 48 h post transfection. Data represent the normalized mean values +/− SEM from at least three independent experiments, each performed in triplicates. One-way ANOVA, multiple comparison correction with Dunnett test. ns = not significant, *P < 0.0332, **P < 0.0021, ***P < 0.0002, ****P < 0.0001. Asterisks indicate the cell lines with H2B-GFP

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