Table 4 List of all cell lines used in the analysis
From: The deregulated microRNAome contributes to the cellular response to aneuploidy
Cell line name | Origin | Full cell line name | Analysis | Remarks |
|---|---|---|---|---|
HCT116 | Koi laboratory | RNA and sRNA sequencing; qPCR | Kindly provided by Minoru Koi | |
HCT116 5/4 | Koi laboratory | RNA and sRNA sequencing; qPCR | Kindly provided by Minoru Koi | |
HCT116 13/3 c2 | MMTC into HCT116 | HCT116 13/3 clone 2 | qPCR | This work |
HCT116 13/3 c3 | MMTC into HCT116 | HCT116 13/3 clone 3 | qPCR | This work |
HCT116 18/3 c1 | MMTC into HCT116 | HCT116 18/3 clone 1 | qPCR | This work |
HCT116 18/3 c2 | MMTC into HCT116 | HCT116 18/3 clone 2 | sRNA sequencing; qPCR | This work |
HCT116 21/3 c1 | MMTC into HCT116 | HCT116 21/3 clone 1 | qPCR | This work |
HCT116 21/3 c3 | MMTC into HCT116 | HCT116 21/3 clone 3 | qPCR | This work |
HCT116* | HCT116 from AATC introduction of H2B-GFP | HCT116 H2B-GFP | qPCR | (Kuffer et al., 2013) [53] |
HCT116* 3/3 c11 | MMTC into HCT116 H2B-GFP | HCT116 H2B-GFP 3/3 clone 11 | sRNA sequencing; qPCR | (Passerini et al., 2016) [9] |
HCT116* 3/3 c13 | MMTC into HCT116 H2B-GFP | HCT116 H2B-GFP 3/3 clone 13 | qPCR | (Passerini et al., 2016) [9] |
HCT116* 5/3 | MMTC into HCT116 H2B-GFP | HCT116 H2B-GFP 5/3 | qPCR | (Stingele et al., 2012) [12] |
HCT116* 8/3 c5 | MMTC into HCT116 H2B-GFP | HCT116 H2B-GFP 8/3 clone 5 | qPCR | (Donnelly et al., 2014) [5] |
HCT116* 8/3 c6 | MMTC into HCT116 H2B-GFP | HCT116 H2B-GFP 8/3 clone 6 | qPCR | (Donnelly et al., 2014) [5] |
HCT116* 8/3 c7 | MMTC into HCT116 H2B-GFP | HCT116 H2B-GFP 8/3 clone 7 | RNA and sRNA sequencing; qPCR | (Donnelly et al., 2014) [5] |
HCT116* 8/3 c8 | MMTC into HCT116 H2B-GFP | HCT116 H2B-GFP 8/3clone 8 | qPCR | (Donnelly et al., 2014) [5] |
RPE1 | Taylor laboratory | RPE1 hTERT | RNA and sRNA sequencing; qPCR | Kindly provided by Steven Taylor |
RPE1 5/3 12/3 | MMTC into RPE1 | RPE1 hTERT 5/3 12/3 | RNA and sRNA sequencing; qPCR | (Stingele et al., 2012) [12] Spontaneous gain of chromosome 12 |
RPE1* | Taylor laboratory | RPE1 H2B-GFP hTERT | RNA and sRNA sequencing; qPCR | Kindly provided by Steven Taylor |
RPE1* 21/3 | MMTC into RPE1 H2B-GFP | RPE1 H2B-GFP hTERT 21/3 | RNA and sRNA sequencing; qPCR | (Stingele et al., 2012 [12]) |
RPE1* 7/3 | MMTC into RPE1 H2B-GFP | RPE1 H2B-GFP hTERT 7/3 | sRNA sequencing; qPCR | This work Spontaneous gain of chromosomes 9,12 |
RPE1* 3/3 | MMTC into RPE1 H2B-GFP | RPE1 H2B-GFP hTERT 3/3 | sRNA sequencing; qPCR | This work |