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Fig. 4 | BMC Genomics

Fig. 4

From: Identification and characterization of microRNAs involved in ascidian larval metamorphosis

Fig. 4

The interactions of identified miRNAs and their target gene prediction. a The interactions of 12 miRNAs and their target genes were visualized as a network using Cytoscape3.4. The target genes displayed in the network included the overlapping ones predicted by miRanda and TargetScan and those genes whose expression levels on performing RNA-Seq were negatively correlated with the expression of miRNAs. Nodes of blue diamonds and pink ovals represented miRNAs and their target genes, respectively. Gradient pink color in the nodes of the target reflects different free energy levels of the miRNA/target duplex (from dark pink to light pink for lower energy to higher energy). Black solid lines between nodes indicated the interrelationships of miRNAs. b Signal pathways related to C. savignyi metamorphosis targeted by miRNAs. Red line rectangles represent target genes; black line rectangles represent genes in a pathway; colored filled rectangles represent different miRNA; green line rounded rectangles represent biological processes; black arrows represent positive regulation; black line-bar represents negative regulation

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