Fig. 4From: Transcriptional profiling of liver in riboflavin-deficient chicken embryos explains impaired lipid utilization, energy depletion, massive hemorrhaging, and delayed featheringA gene interaction network involving several upstream regulators and their direct target genes involved in “Cellular Growth and Proliferation” (a). Ingenuity® Up-stream Regulator Analysis predicts activation of two transcription factors, JUN proto-oncogene, AP-1 transcription factor subunit (JUN) and thyroid hormone receptor beta (THRB), and their direct target genes (b). These gene networks were identified in a combined dataset of all AR-DE genes found in the liver at e13 and e15 (see Fig. 2b). The target genes were either upregulated in riboflavin-rescued (Rf+; red-colored symbols) or expressed higher in riboflavin-deficient (Rf-; green-colored symbols) embryo livers. IPA predicts that the two up-stream regulators are “activated”, which would lead to either activation (orange arrows) or inhibition (blunt blue edges) of their respective direct target genesBack to article page