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Fig. 5 | BMC Genomics

Fig. 5

From: Identification, characterization and gene expression analyses of important flowering genes related to photoperiodic pathway in bamboo

Fig. 5

In silico study on the molecular interactions between individual BtFT and Os14-3-3 proteins. a Detailed sequence analysis of BtFT protein regions responsible for interacting with 14-3-3 protein. Seven amino acids conserved for 14-3-3 interaction are highlighted in grey. Amino acids not conserved in B. tulda are highlighted in black. Sequences used are: OsHd3a: Os06g06320.1, OsRFT1: Os06g06300.1, BdFT: Bradi1g48830.1, PhFT: PH01002288G0050, PmFT1: AB498760.1, PmFT2: AB240578.1, PmFT3: AB498761.1, PmFT4: AB498762.1, BtFT1: KT003820, BtFT2: KT003821, BtFT3: KU726232, BtFT4: KX290774, PvFT1: Guo et al. (2015), PvFT2: Guo et al. (2015), HvFT1: DQ100327, HvFT2: DQ297407.1, HvFT3: DQ411319, HvFT4: DQ411320, TaFT: DQ890162.1, ZmFT: EU241924, SbFT1: XP_002436509.1, SbFT8: XP_002456354.1, SbFT10: Sb09g025760. b Conserved interaction pattern between BtFT and 14-3-3. Given more than 86% homology with each other as well as rice counterpart Hd3a, all BtFT proteins (1-4) are almost perfectly superimposable to each other. Their interaction pattern with 14-3-3 also remains mostly conserved. c Surface analysis showing interdigitated interface between a pair of BtFT and 14-3-3. d Conserved salt bridge interactions between BtFT and 14-3-3. Asp208-Arg130 and Glu212-Arg62 salt bridges could be essential for BtFT’s interaction not only with 14-3-3 but also with FD. e Difference in hydrophobic cavity lining BtFT proteins. Subtle alterations e.g. Phe101 to Ile101in BtFT1, 2 and Phe64 to Leu64 in BtFT3, 4 might alter the specificity of BtFT1,2/3,4 interaction with 14-3-3

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