Fig. 1

High expression of DLG2 in IFNβ-producing pDCs. (a) Analysis of Dlg2 expression in splenic pDCs. IFNβ/YFP⁺ and IFNβ/YFP^— splenic pDCs were ex vivo FACS sorted from IFNβ^mob/mob mice 6 h after i.v. injection with CpG. Dlg2 (white bars) and Ifnb (black bars) mRNA expression was analysed by quantitative RT-PCR. (b) Dlg2 expression in in vitro BM-derived pDCs. BM-derived DCs were generated from IFNβ^mob/mob mice in Flt3L cultures. IFNβ/YFP⁺ and IFNβ/YFP^— BM-derived pDCs were FACS sorted 6 h after stimulation with CpG. Dlg2 (white bars) and Ifnb (black bars) mRNA expression was analysed by quantitative RT-PCR. Data shown in (a) and (b) is fold mRNA quantity in IFNβ-producing pDCs relative to IFNβ non-producers. Data shown is mean ± standard error of mean (SEM) from four independent experiments (each sample is pooled from 12 (a) or 6 (b) mice. Statistical differences between IFNβ/YFP⁺ and IFNβ/YFP^— were analysed by two tailed, unpaired t test. ns: P > 0.05, *: P < 0.05, **: P < 0.01, ***: P < 0.001. (c) Immunofluorescence images of DLG2 expression in BM-derived Flt3L cultured pDCs left untreated (naïve; upper panel) and 24 h after CpG (CpG; lower panel) stimulation. Cells were stained with antibodies against DLG2 and mPDCA-1 and a YFP-crossreacting antibody against GFP. Data shown is one representative experiment out of three independent experiments with comparable results