Fig. 5

The effect of ectopic expression of Dlg2 isoforms on Ifnb expression. (a). Effect of IRF7 activation on Ifnb promoter activity. IRF7 or TBK1 were expressed alone or in combination in the presence of Ifnb reporter plasmid. Control (Cont.) shows the basal activity of Ifnb promoter in NIH3T3 cells. (b). Effect of expression of Dlg2 isoforms on Ifnb promoter activity. Increasing amounts of Dlg2 isoforms or TPI gene were transiently transfected along with Irf7, Tbk1 encoding plasmids and an Ifnb reporter vector in NIH3T3 cells. Ifnb promoter activity was measured from cell lysates one day after transfection. Data shown are ratios of relative light unites of Firefly luciferase (under the control of the Ifnb promoter) / Renila luciferase (under the control of the CMV promoter) from one representative experiment performed in quadruplicates out of three independent experiments. Data shown are the means ±SEM. Significance was analyzed by either One-way ANOVA followed by Bonferroni’s Multiple Comparison Test (a) or Two-way ANOVA followed by Bonferroni posttests (b). ns: P > 0.05, *: P < 0.05, **: P < 0.01, ***: P < 0.001