Table 1 Primer used for isotype specific RT-PCR
Name | Exon | Sequences (5′-3′) |
|---|---|---|
Dlg2α1 fwd | α1 | CTGAGCTCTCACTCAGTGCCTTC |
Dlg2α2 fwd | α2 | AGCTGCCGCTCTGTCTAGGCTG |
Dlg2δ fwd | δ | GGGAGGAAGCCTTTCTATGCAG |
Dlg2rev | 11 | CGGTGGCCCATAAGGATCAGT |
Dlg2α0/β fwd | 4 | AAGGCAAATGCCCAGCCCAG |
Dlg2α0/β rev | 9 | TAGAGCCGGCTTCCTTGAG |
Dlg2γ fwd | γ | GTGAAGAAGCTATGCAACACGCGT |
Dlg2γ rev | 14 | CGAGTTGCAGTACTGTGCTGG |
Dlg2ε fwd | ε | GCCAACTGGATGTGTGTGAGCCG |
Dlg2ε rev | 9 | CACAACAGTCTCCAATATGGGTCGC |
Dlg2ζ1 fwd | ζ1 | AGCTGGCTGTGTTTCCAGTGCC |
Dlg2ζ2 fwd | ζ2 | TCCCCAGAGCCCATATAGAGCCA |
Dlg2η1 fwd | η1 | ACACAACAGCCCCCAAACCAC |
Dlg2η2 fwd | η2 | ACCGGACAGGAGACAGGAGA |
Dlg2η rev | 17 | TGACATACAGGGAGCGTTTCT |
Dlg2 E18 fwd | 18 | AGAAGGGTCACACTAGATGG |
Dlg2 E19 fwd | 19 | GTGGAAAGAAAGGAGCGTG |
Dlg2 E20 fwd | 20 | CCATTCTACAAGAACAAGGAGC |
Dlg2 E21 fwd | 21 | ATTAGGTGACGACGGTTATGG |
Dlg2 E20 rev | 20 | ATCACTGGTTTCCTGCTCAC |
Dlg2 E21 rev | 21 | GAGTCTTTGTTCCATAACCGTC |
Dlg2 E22 rev | 22 | AGCTACTTTCGCTATCGCTG |
Dlg2 E23 rev | 23 | GCCTCGTGACAGGTTCATAG |