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Table 1 Species specific primers used to amplify mtDNA of Anodonta cygnea using LR-PCR. FOR = forward, REV = reverse, Tm = melting temperature

From: The complete mitochondrial genome of the hermaphroditic freshwater mussel Anodonta cygnea (Bivalvia: Unionidae): in silico analyses of sex-specific ORFs across order Unionoida

Primer Name

Sequence 5′ – 3′

Tm (°C)

Target Gene

ACP3 short FOR

CCAGCTAAAACAGGCAAAGC

63.7

cox1

ACP3 short REV

CGGGGTCTTTTCGTCTACCT

64.2

rrnl

ACP3 long FOR

CCTCGATGTTGGCTTAAGGA

64.0

rrnl

ACP3 long REV

GGAGGTTATTGGGGGATGAT

63.6

cox1