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Table 3 Z-test of selection results for purifying selection (Ho: dn = ds, Ha: dn < ds) and positive selection (Ho: dn = ds, Ha: dn > ds) on alignments of (A) complete nad5 from 12 species with 543 positions, and (B) nad5 sequences corresponding to a putative translocated portion of nad5 from Anodonta cygnea (MF781083) from 12 species with 45 positions in the analysis. Alignments by ClustalW [36] and Z-tests were conducted in MEGA7 [37] using the Kumar method, 1000 bootstraps, and pairwise deletion. Statistically significant results are highlighted in bold using α = 0.05

From: The complete mitochondrial genome of the hermaphroditic freshwater mussel Anodonta cygnea (Bivalvia: Unionidae): in silico analyses of sex-specific ORFs across order Unionoida

A B
Test P-value Test statistic Test P-value Test statistic
Purifying selection < 0.001 14.932 Purifying selection 1.000 −6.456
Positive selection 1.000 −14.495 Positive selection < 0.001 6.842