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Table 3 RT-qPCR results and correlation. Values in parentheses represent Fold Changes (FC)

From: Lowered dietary phosphorus affects intestinal and renal gene expression to maintain mineral homeostasis with immunomodulatory implications in weaned piglets

Tissue Gene name Microarray qPCR# Correlation##
H vs L H vs M M vs L H vs L H vs M M vs L
Kidney CYP27A1 H < L
(2.11)
H < M
(1.45)
M < L
(1.46)
H < L***
(2.83)
H < M**
(1.60)
M < L**
(1.77)
0.94
NFATC2 H < L
(1.33)
H > M
(1.13)
M < L**
(1.51)
H < L*
(1.76)
H > M
(1.04)
M < L*
(1.82)
0.66
PTH1R H > L
(1.14)
H < M
(1.09)
M > L
(1.26)
H > L
(1.03)
H < M
(1.20)
M > L
(1.24)
0.62
SLC34A3 H > L
(1.04)
H < M
(1.26)
M > L
(1.31)
H < L
(1.30)
H < M*
(1.40)
M > L
(1.07)
0.61
SPP1 H < L
(1.48)
H > M
(1.46)
M < L***
(2.16)
H < L*
(2.02)
H > M
(1.19)
M < L**
(2.41)
0.92
VDR H > L***
(1.75)
H < M
(1.05)
M > L***
(1.84)
H > L***
(1.75)
H < M
(1.08)
M > L***
(1.88)
0.76
Jejunum CYP24A1 H < L***
(7.69)
H < M
(1.48)
M < L***
(5.19)
H < L**
(222.14)
H < M
(2.79)
M < L*
(79.59)
0.89
C1QC H > L**
(1.70)
H < M
(1.18)
M > L***
(2.02)
H > L**
(1.79)
H < M
(1.05)
M > L**
(1.88)
0.83
C1R H > L**
(1.85)
H < M
(1.19)
M > L***
(2.24)
H > L**
(2.19)
H < M
(1.15)
M > L**
(2.52)
0.93
C1S H > L***
(3.08)
H < M
(1.19)
M > L***
(4.36)
H > L**
(2.48)
H < M
(1.34)
M > L***
(3.33)
0.89
C7 H > L
(2.02)
H < M
(1.18)
M > L**
(2.47)
H > L*
(2.64)
H < M
(1.14)
M > L**
(3.00)
0.94
MMP2 H > L***
(2.20)
H > M
(1.18)
M > L**
(1.88)
H > L**
(4.05)
H > M
(1.21)
M > L**
(3.35)
0.92
  1. Significance level was set at ***p < 0.001; **p < 0.01, *p < 0.05 and q < 0.2 for results from microarray and qPCR analyses. Fold Changes are stated in parentheses. # - Values were calculated by factorial normalization on RPL32 expression values. ## - Correlation of normalized values was calculated by Spearman; the significance level for correlation was set at p < 0.01. Scatterplots of jejunal and renal transcripts are displayed in Additional file 6