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Table 3 RT-qPCR results and correlation. Values in parentheses represent Fold Changes (FC)

From: Lowered dietary phosphorus affects intestinal and renal gene expression to maintain mineral homeostasis with immunomodulatory implications in weaned piglets

Tissue

Gene name

Microarray

qPCR#

Correlation##

H vs L

H vs M

M vs L

H vs L

H vs M

M vs L

Kidney

CYP27A1

H < L

(2.11)

H < M

(1.45)

M < L

(1.46)

H < L***

(2.83)

H < M**

(1.60)

M < L**

(1.77)

0.94

NFATC2

H < L

(1.33)

H > M

(1.13)

M < L**

(1.51)

H < L*

(1.76)

H > M

(1.04)

M < L*

(1.82)

0.66

PTH1R

H > L

(1.14)

H < M

(1.09)

M > L

(1.26)

H > L

(1.03)

H < M

(1.20)

M > L

(1.24)

0.62

SLC34A3

H > L

(1.04)

H < M

(1.26)

M > L

(1.31)

H < L

(1.30)

H < M*

(1.40)

M > L

(1.07)

0.61

SPP1

H < L

(1.48)

H > M

(1.46)

M < L***

(2.16)

H < L*

(2.02)

H > M

(1.19)

M < L**

(2.41)

0.92

VDR

H > L***

(1.75)

H < M

(1.05)

M > L***

(1.84)

H > L***

(1.75)

H < M

(1.08)

M > L***

(1.88)

0.76

Jejunum

CYP24A1

H < L***

(7.69)

H < M

(1.48)

M < L***

(5.19)

H < L**

(222.14)

H < M

(2.79)

M < L*

(79.59)

0.89

C1QC

H > L**

(1.70)

H < M

(1.18)

M > L***

(2.02)

H > L**

(1.79)

H < M

(1.05)

M > L**

(1.88)

0.83

C1R

H > L**

(1.85)

H < M

(1.19)

M > L***

(2.24)

H > L**

(2.19)

H < M

(1.15)

M > L**

(2.52)

0.93

C1S

H > L***

(3.08)

H < M

(1.19)

M > L***

(4.36)

H > L**

(2.48)

H < M

(1.34)

M > L***

(3.33)

0.89

C7

H > L

(2.02)

H < M

(1.18)

M > L**

(2.47)

H > L*

(2.64)

H < M

(1.14)

M > L**

(3.00)

0.94

MMP2

H > L***

(2.20)

H > M

(1.18)

M > L**

(1.88)

H > L**

(4.05)

H > M

(1.21)

M > L**

(3.35)

0.92

  1. Significance level was set at ***p < 0.001; **p < 0.01, *p < 0.05 and q < 0.2 for results from microarray and qPCR analyses. Fold Changes are stated in parentheses. # - Values were calculated by factorial normalization on RPL32 expression values. ## - Correlation of normalized values was calculated by Spearman; the significance level for correlation was set at p < 0.01. Scatterplots of jejunal and renal transcripts are displayed in Additional file 6