Fig. 7

Alternative and differential splicing of HHV-6B U79 transcripts in Sup-T1 versus MOLT3 cells. Strand-specific RNA sequencing reveals three additional spliceoforms of the U79 gene in HHV-6B Z29 strain cultured in Sup-T1 cells compared with the Z29 reference annotation in NC_000898 (a). Reads depicted in orange are positive-sense reads, while negative-sense reads are shown in blue. The highlighted peptide from the U79a2 transcript in red was confirmed by shotgun proteomics of the Sup-T1 cultured HHV-6B. While in SupT1 four total spliceoforms are found (b), in MOLT3 cells, only two forms of splicing in U79 are detected (c)