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Table 2 Mean read length and number of circular consensus sequences (CCSs) for COI from the four libraries in three data partitions. The percentage values (99%, 99.9%, 99.99%) indicate the proportion of bases in each CCS that is predicted to match its template based on Pacific Bioscience’s model of the circular sequencing process

From: A Sequel to Sanger: amplicon sequencing that scales

Library

Mean Read

Number of Reads

PCR Protocol

CCS 99%

CCS 99.9%

CCS 99.99%

99.99%/99%

1

11.6 kb

518,155

1

198,173

154,684

60,116

0.30

2

14.3 kb

608,685

1

334,556

243,020

95,225

0.28

3

13.5 kb

369,904

2

221,289

131,654

48,625

0.22

4

15.7 kb

384,274

2

223,325

139,292

51,683

0.23

Mean

13.8 kb

470,254

–

244,336

167,163

63,912

–