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Table 2 Mean read length and number of circular consensus sequences (CCSs) for COI from the four libraries in three data partitions. The percentage values (99%, 99.9%, 99.99%) indicate the proportion of bases in each CCS that is predicted to match its template based on Pacific Bioscience’s model of the circular sequencing process

From: A Sequel to Sanger: amplicon sequencing that scales

Library Mean Read Number of Reads PCR Protocol CCS 99% CCS 99.9% CCS 99.99% 99.99%/99%
1 11.6 kb 518,155 1 198,173 154,684 60,116 0.30
2 14.3 kb 608,685 1 334,556 243,020 95,225 0.28
3 13.5 kb 369,904 2 221,289 131,654 48,625 0.22
4 15.7 kb 384,274 2 223,325 139,292 51,683 0.23
Mean 13.8 kb 470,254 244,336 167,163 63,912