Fig. 1

Workflow of the modified sequence capture method. Following fragmentation of the genomic DNA, a SureSelect Methyl-Seq library was constructed and hybridised to custom baits. Bait/target hybrids were bound to streptavidin beads, which were then washed to remove non- specifically bound DNA fragments. Target enriched DNA was eluted from the streptavidin beads and the eluate divided; ~ 3/4 of the eluate was bisulphite converted and then amplified, ~ 1/4 was neutralised, purified and then amplified. The quality of the purified libraries was assessed prior to sequencing