Fig. 2From: METHimpute: imputation-guided construction of complete methylomes from WGBS dataConceptual overview of METHimpute. a Cytosines on the sequenced genome are assumed to be either unmethylated or methylated. b Bisulphite-sequencing and alignment yields methylation levels for each cytosine, i.e. the number of reads showing methylation divided by the total number of reads. c Emission densities for each state are obtained with a binomial test with state-specific parameters. Note that "imputed" cytosines, i.e. cytosines without any reads, are treated identically as all other cytosines. However, since the emission densities for all states are 1 for imputed cytosines, the methylation status call is purely driven by the neighborhood of cytosines. d Model fitting yields posterior probabilities for methylation status calls. e Inferred methylation status calls and methylation levelsBack to article page