Fig. 4
Quantification (log2 of fold-change; mean ± SEM) of the mRNA profiles in granulosa cells after a Long (7-day) compared to conventional (4-day, reference group) FSH treatment. Cells were obtained from the FSH-stimulated follicles 24 h after exogenous LH treatment in both groups. Bars extending to the right and left of zero represent up- and down-regulated genes, respectively, in the Long FSH group. RT-PCR data analysis was performed using REST 2009 program. Light grey bars represent expression of transcript in microarray experiment while dark grey bars represent expression of the same transcript obtained by RT-PCR. Asterisk (*) by the side of a bar represent statistical difference (P < 0.1) between long FSH in relation to the conventional FSH in RT-PCR experiments