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Table 1 Primers used for RT-PCR

From: Transcriptome analysis of granulosa cells after conventional vs long FSH-induced superstimulation in cattle

Genes Strand Primer sequence Annealing temperature (°C)
  Forward 5’-CATGAGATGGCAGTCAATTTGT-3’ 53.6
EIF2B2 Reverse 5’-CTTGAACATAGGAGCACAGACG-3’ 55.5
  Forward 5’-TGTGTCCCTCTTGCTGAGTTT-3’ 56.4
SF3A1 Reverse 5’-ATTCCTGGTTTCACGTCTCCTA-3’ 55.5
  Forward 5’-TGGACTCAGAAGTATGCGATGT-3’ 55.8
UBE2D2 Reverse 5’-CTTCTCTGCTAGGAGGCAATGT-3’ 56.6
  Forward 5’-AGTGTTCCCTCTTGGAAAATGA-3’ 60
NTS Reverse 5’-TCTTCCTGAATCAACTCCCAGT-3’ 60.1
  Forward 5’-AAAGCTCTAGGGGGTTCTCGT-3’ 56.3
PTGS2 Reverse 5’-TGTCAGCACATCCAGGGTAA-3’ 56
  Forward 5’-GGCAGACTCACAGGCTTCAATATC-3’ 57.6
PTX3 Reverse 5‘-CCTTCTCCAGTCTCCCTTTCAACT-3’ 58.2
  Forward 5’-AAAGCCGCAGATCACCACAGAA-3’ 59.3
RGS2 Reverse 5’-TCCAGCTTGAGACACACCACAT-3’ 58.6
  Forward 5’-CGACTTCATCGAACACATCCTTCG-3’ 57.7
CCND2 Reverse 5’-CTATTCAGCAGCACCACCTCAATC-3’ 57.8
  Forward 5’-ACGCAAAGTTCTCGCAAGCTCA-3’ 59.7
LRP8 Reverse 5’-TGCC ATTTCCTCCTCAAACAGG-3’ 57.6
  Forward 5’-CCAAAGGATGTACCCAACTCTC-3’ 59.8
INHBA Reverse 5’-GTCCGATGTCGTCCTCTATCTC-3’ 60.7
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