Table 1 Primers used for RT-PCR
Genes | Strand | Primer sequence | Annealing temperature (°C) |
|---|---|---|---|
|  | Forward | 5’-CATGAGATGGCAGTCAATTTGT-3’ | 53.6 |
EIF2B2 | Reverse | 5’-CTTGAACATAGGAGCACAGACG-3’ | 55.5 |
|  | Forward | 5’-TGTGTCCCTCTTGCTGAGTTT-3’ | 56.4 |
SF3A1 | Reverse | 5’-ATTCCTGGTTTCACGTCTCCTA-3’ | 55.5 |
|  | Forward | 5’-TGGACTCAGAAGTATGCGATGT-3’ | 55.8 |
UBE2D2 | Reverse | 5’-CTTCTCTGCTAGGAGGCAATGT-3’ | 56.6 |
|  | Forward | 5’-AGTGTTCCCTCTTGGAAAATGA-3’ | 60 |
NTS | Reverse | 5’-TCTTCCTGAATCAACTCCCAGT-3’ | 60.1 |
|  | Forward | 5’-AAAGCTCTAGGGGGTTCTCGT-3’ | 56.3 |
PTGS2 | Reverse | 5’-TGTCAGCACATCCAGGGTAA-3’ | 56 |
|  | Forward | 5’-GGCAGACTCACAGGCTTCAATATC-3’ | 57.6 |
PTX3 | Reverse | 5‘-CCTTCTCCAGTCTCCCTTTCAACT-3’ | 58.2 |
|  | Forward | 5’-AAAGCCGCAGATCACCACAGAA-3’ | 59.3 |
RGS2 | Reverse | 5’-TCCAGCTTGAGACACACCACAT-3’ | 58.6 |
|  | Forward | 5’-CGACTTCATCGAACACATCCTTCG-3’ | 57.7 |
CCND2 | Reverse | 5’-CTATTCAGCAGCACCACCTCAATC-3’ | 57.8 |
|  | Forward | 5’-ACGCAAAGTTCTCGCAAGCTCA-3’ | 59.7 |
LRP8 | Reverse | 5’-TGCC ATTTCCTCCTCAAACAGG-3’ | 57.6 |
|  | Forward | 5’-CCAAAGGATGTACCCAACTCTC-3’ | 59.8 |
INHBA | Reverse | 5’-GTCCGATGTCGTCCTCTATCTC-3’ | 60.7 |