Fig. 1

Normalized expression level of PyOLE-1 gene in different genotypes in temperature stress. (a, b, c) Relative quantification of PyOLE-1 gene expression using the best stable reference genes (MAP, UBC and CGS1) and the least stable genes (PPK, DPE2 and eif4A) under temperature stress in RZ58. d, e, f Relative quantification of PyOLE-1 gene expression using the best stable reference genes (MAP, UBC and CGS1) and the least stable genes (PPK, DPE2 and eif4A) under temperature stress in S21. g, h, i Relative quantification of PyOLE-1 gene expression using the best stable reference genes (MAP, UBC and CGS1) and the least stable genes (PPK, DPE2 and eif4A) under temperature stress in PyC-1. The average Ct value was calculated from three biological and technical replicates and used for relative expression analyses. Error bars indicate standard errors. The statistical significance was showed by one or two asterisks (P value is lower than 0.05 or 0.01) respectively