Transcriptome-wide identification of optimal reference genes for expression analysis of Pyropia yezoensis responses to abiotic stress

Table 2 Genes and primer sets for RT-qPCR

Gene name	Gene ID	Gene symbol	Prime sequence (forward/reverse)	Size(bp)	RT-qPCR Efficiency^a	Error^b
Methionyl aminopeptidase	TRINITY_DN10053_c0_g1_i1	MAP	TGGGTAGGAAGTGGGGCT	200	1.946	0.0793
			CGTGGTAGGTCGGTAGGC
AAA-type ATPase	TRINITY_DN1508_c0_g1_i1	ATPase	CGACGAGATTGACGCA	132	2.014	0.0744
AAA-type ATPase			GTCGCCCCAATCACAA
Cystathionine gamma-synthase, Rhodoplastic	TRINITY_DN3362_c1_g1_i1	CGS1	CTACGGACACCAAGAAACG	106	1.947	0.121
			CTCGGTTGGCTGGGTAA
polyphosphate kinase, partial	TRINITY_DN8109_c0_g1_i1	PPK	GTGTCTGGTCCACGCTC	160	1.98	0.101
			CACGAGGTGCTGACTGAG
Disproportionating Enzyme type 2	TRINITY_DN10209_c0_g1_i1	DPE2	CACGGAAGGTAGGAAAGGA	204	1.992	0.0582
			AGGTGGGTGTTGGGGTT
fumarate hydratase precursor	TRINITY_DN7986_c2_g1_i1	FHP	TAATGTGCGAAAAGGCGG	146	1.973	0.117
			CGTGAACAAGTCCCAGTCCT
ubiquitin-conjugating enzyme	TRINITY_DN9056_c0_g3_i1	UBC	CGCTGACCGTTTCCAAG	112	1.99	0.0598
			CGACTGCGGTTGGACTT
elongation factor	TRINITY_DN9169_c0_g1_i2	EF	TGCGAGTCAACCAGGAG	175	1.96	0.153
			GCCTCAAGAAACACCCTA
translation initiation factor 4 A	TRINITY_DN16387_c0_g1_i1	eif4A	ATGGACCAGAAGGACCG	139	1.951	0.144
			TCGTGGGCAGGTCATAG

^a The RT-qPCR amplification efficiency for each primer were determined by LightCycle®480 gene scanning software (version 1.5)
^b The Error value of amplification efficiency for each primer were determined by LightCycle®480 gene scanning software (version 1.5) and an acceptable vaule should be< 0.2

ISSN: 1471-2164