Fig. 4From: A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome highlights the challenges associated with draft genomes and gene prediction in plantsExamples of genes with transcription, and no transcription in intronic regions highlighting some of the challenges associated with manual annotation. a RNA-Seq reads in a single intron of gene Acc00023. b and c a comparison of RNA-Seq reads in two independent RNA-Seq libraries of gene Acc00022. B) RNA-Seq from a root library showing transcription across the intron and C) RNA-Seq from a flower library showing no intronic RNA-Seq reads. d Transcription between gene models Acc00024 and Acc00025, which could not be constructed into a gene model; the Actinidia chinensis ‘Hongyang’ predicted model line-up Achn243511 is shown in blueBack to article page