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Fig. 4 | BMC Genomics

Fig. 4

From: A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome highlights the challenges associated with draft genomes and gene prediction in plants

Fig. 4

Examples of genes with transcription, and no transcription in intronic regions highlighting some of the challenges associated with manual annotation. a RNA-Seq reads in a single intron of gene Acc00023. b and c a comparison of RNA-Seq reads in two independent RNA-Seq libraries of gene Acc00022. B) RNA-Seq from a root library showing transcription across the intron and C) RNA-Seq from a flower library showing no intronic RNA-Seq reads. d Transcription between gene models Acc00024 and Acc00025, which could not be constructed into a gene model; the Actinidia chinensis ‘Hongyang’ predicted model line-up Achn243511 is shown in blue

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