Fig. 3From: A machine learning model to determine the accuracy of variant calls in capture-based next generation sequencingHigh confidence, low allele fraction variant. Example of a true positive prediction of a low allele fraction variant, ATM (c.6491A > G). Top: NGS reads, visualized in IGV [18, 19] showing the variant was detected in reads of both directions. Bottom: Sanger sequencing chromatogram, showing a detected allele fraction of ~ 25%Back to article page