Fig. 1

Neuronal differentiation in retinal organoids. a-b Retinal organoids on day 23 of differentiation displaying (a) phase bright neural retinal tissue and (b) retinal pigmented epithelium (RPE). c-f Immunostained cryosections of retinal organoids on day 90 of differentiation counterstained with Hoechst demonstrating the laminar organisation of retinal tissue and presence of (c) Pax6+ retinal progenitors, inner retinal neurons and (d) HuC/D+ retinal ganglion cells at the basal aspect, and (e, f) outer retinal phenotypes including Crx + and recoverin+ photoreceptors towards the apical surface. GCL = ganglion cell layer, ONL = outer nuclear layer. Scale bars; a, b = 200 μm; c, d = 50 μm; e, f = 20 μm