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Table 1 Percentages of isomiRs (# of the isomiRs showing the indicated modification / # of total reads) of the miR-34 family activated by p53

From: Robust Sub-nanomolar Library Preparation for High Throughput Next Generation Sequencing

 

5′ modifications

3′ modifications

nucleotide substitution

 

Illumina

sub-nanomolar

Illumina

sub-nanomolar

Illumina

sub-nanomolar

hsa-miR-34a-5p

4.2%

3.2%

58.3%

58.0%

9.6%

9.8%

hsa-miR-34a-3p

~

~

~

~

~

~

hsa-miR-34b-5p

95.1%

96.3%

91.8%

92.6%

6.6%

7.4%

hsa-miR-34b-3p

73.4%

66.7%

98.7%

100.0%

7.6%

8.3%

hsa-miR-34c-5p

0.9%

1.4%

6.1%

5.2%

2.6%

2.3%

hsa-miR-34c-3p

~

~

~

~

~

~

  1. ~: # of -3p reads much lower than # of -5p reads to show reliable ratios