Fig. 3

Clustering analysis of the Calycin/lipocalin gene family with CLANS in the three-Triatoma and R. prolixus. 2D representation of the clusters obtained through the CLANS analysis. A large calycin expansion was identified in the three-Triatoma species, but more remarkable in T. infestans. Although such expansion involves typical salivary lipocalins (clades I, II, III, IV, V, VI and VII, and nitrophorins) two novel non-salivary clade were identified: the Intestinal clade (purple) and the FABP (pink). The colored dots in the graph shown indicate sequences included in each calycin group, while black dots represent divergent sequences that do not cluster within any predefined clade. A detailed description of the calycin coding transcripts in Triatoma is found in Additional file 2