Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors

Table 1 Sequencing metrics

	T. dimidiata		T. infestans		T. pallidipennis
Raw sequencing
Raw reads	358,962		559,962		626,401
Filtered reads (PRINTSEQ)	237,226	66.1%	347,620	62.1%	421,667	67.3%
Number of bases (Mbps)	112.8		164.6		202.6
Assembly
Aligned reads	173,525	73.3%	161,674	46.6%	353,083	83.8%
Aligned bases (Mbps)	82.3	73.1%	74.2	45.1%	167.2	82.6%
Assembled reads	140,289	59.3%	124,306	35.8%	291,051	69.1%
Partially assembled reads	33,138	14.0%	37,334	10.8%	61,847	14.7%
Singletons	56,397	23.8%	174,467	50.3%	54,964	13.1%
Isogroups	4847		3904		5148
Average contig count	1.6		1.7		1.8
Average isotig count	1.3		1.2		1.3
Largest isotig count	15		30		18
Number of isotigs	6212		4856		6476
Average contig count	1.6		1.5		1.5
Largest contig count	7		9		9
Isotigs with one contig	3824		3232		4169
Number of bases (Mbps)	5.2		4.1		5.8
Average isotig size	840		841		893
N50 isotig size	880		871		921
Largest isotig size	6814		3682		4465
Q40 plus bases (Mbps)	3.5	95.8%	2.9	95.9%	4.0	95.4%
< Q39 bases (Mbps)	0.2	4.2%	0.1	4.1%	0.2	4.6%
full_dataset
Total sequences	29,024		35,629		31,175
Contigs/isotigs	6217	21%	4886	14%	6530	21%
Singletons	22,807	79%	30,743	86%	24,645	79%
Total bases (Mbps):	16.9		20.9		18.2
% G + C	33.73	± 5.48%	35	± 6.12%	33.7	± 5.75%
nr_dataset (1 isotig per isogroup)
Total sequences	27,652		34,646		29,789
Contigs/isotigs (isotig_dataset)	4845	18%	3903	11%	5144	17%
Singletons	22,807	82%	30,743	89%	24,645	83%
Total bases (Mbps):	15.8		20.1		17.1

ISSN: 1471-2164