Skip to main content
Fig. 2 | BMC Genomics

Fig. 2

From: Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions

Fig. 2

Bidirectional promoter possesses distinct epigenetic histone marks. Figure depicts ChIP analysis of the genomic locus harboring the bidirectional gene pair NFYA/OARD1 which includes 1 Kb upstream and downstream regions from the TSS of both genes. Occupancy profile of NFYA and OARD1 in Jurkat cells for Histone modifications associated with transcription activation (H3K4me3) and transcription elongation (H3K79me3) was deduced along with RNA polII phosphorylated at serine 5 using ChIP assay as described in ‘Methods’. INTA and INTB denote the two ends of the intergenic region (TSS of OARD1 and NFYA respectively), A - E indicate the location of the ChIP-qPCR primers used to map the histone modifications

Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us