Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions

Table 1 List of bidirectional promoters tested using the pDR vector

Gene Pair	Cloned region (hg19)	Length (bp)	Clone name
FOXM1-RHNO1	chr12:2986293–2,986,670	380	BDP-S1/AS1
LOC105369155-TMEM208	chr16:67260620–67,261,120	503	BDP-S2/AS2
TP53-WARP53	chr17:7590787–7,591,798	1000	BDP-S3/AS3
TMEM234-EIF3I	chr1:32687713–32,688,023	310	BDP-S4/AS4
CIB1-NGRN	chr15:90777156–90,777,587	431	BDP-S5/AS5
HNRNPA2B1-CBX3	chr7:26240385–26,241,390	1005	BDP-S6/AS6
CBX5-HNRNPA1	chr12:54653296–54,654,074	778	BDP-S7/AS7
GFM2-NSA2	chr5:74062655–74,063,004	350	BDP-S8/AS8

The IDs of genes within the bidirectional pair and their chromosomal locations as well as the length of intergenic distance (= bidirectional promoter) are listed. The clones in sense to eGFP were named as S1-S8 and the same clone when in sense to mCherry was named AS1-AS8 (Fig. 1, Additional file 1: Figure S1)

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