The dehydration stress of couch grass is associated with its lipid metabolism, the induction of transporters and the re-programming of development coordinated by ABA

Anna Janská; Pavel Svoboda; Vojtěch Spiwok; Ladislav Kučera; Jaroslava Ovesná

doi:10.1186/s12864-018-4700-3

Table 3 Genes stimulated or inhibited by dehydration within couch grass rhizomes selected for qRT-PCR analysis, sequences of the primers and amplification efficiencies

From: The dehydration stress of couch grass is associated with its lipid metabolism, the induction of transporters and the re-programming of development coordinated by ABA

ID^a	AGI^b	Name^c	Primer sequence	Efficiency (%)^d
Contig15047_at	AT5G20730.2	NPH4_F	atcctatcccctcaagaagtgcaaa	93
Contig15047_at	AT5G20730.2	NPH4_R	tggtcgtaacgaggcttccaagtat	93
Contig1223_at	AT3G53420.2	PIP_F	agtacgtcctgagggcgagtg	92
Contig1223_at	AT3G53420.2	PIP_R	cacgatccgagccatatcacactgat	92
Contig14720_s_at	AT1G34260.1	PI3PK_F	gagtttgtacttggcatcatcgact	95
Contig14720_s_at	AT1G34260.1	PI3PK_R	aaccgttaggaaatacttggccatg	95
HVSMEf0022D18r2_s_at	AT3G22600.1	LTPG5_F	gatcgggttggcgcgcataca	92
HVSMEf0022D18r2_s_at	AT3G22600.1	LTPG5_R	atgcatgtcacggtacaacaaatgga	92
Contig10474_at	AT1G80950.1	PLGAC_F	gttgctctttcctgagggcac	108
Contig10474_at	AT1G80950.1	PLGAC_R	aaaatgactggttgtactggtgctc	108
Contig3810_at	AT1G09350.1	WSI76_F	tacgtgcaagcacacggttgg	97
Contig3810_at	AT1G09350.1	WSI76_R	acgtttcagccatgcatacgtgtacg	97
Contig14329_at	AT1G04870.2	PRMT10_F	ttgatgactccatctccgagagtaa	94
Contig14329_at	AT1G04870.2	PRMT10_R	atccatatccataagccggtgattc	94
Contig10182_at	AT1G15520.1	ABC_F	tcagccctattgcatggacactcaa	94
Contig10182_at	AT1G15520.1	ABC_R	gctactacccacaggaagtcgtgat	94
HT11N18r_s_at	AT1G78390.1	NCED_F	cttattaggcataggagatccccgg	94
HT11N18r_s_at	AT1G78390.1	NCED_R	tgaagcaagtgtgagctaactgaat	94
Contig1708_s_at	AT4G01985.1	DHN6_F	agcacaagaccggtggcatcct	103
Contig1708_s_at	AT4G01985.1	DHN6_R	tccttgttaccgccggggagct	103
HW09B04u_at	AT4G39670.1	GLTP_F	cgttccatagctgggcaatccaga	108
HW09B04u_at	AT4G39670.1	GLTP_R	acagagcaatcagtttcgttgagcc	108
Contig2406_at	AT3G15670.1	WRAB1_F	ttgcctttgatttgatggtactcgtgt	97
Contig2406_at	AT3G15670.1	WRAB1_R	gtgccacctttcgactgtcctc	97
Contig1718_s_at	AT3G50980.1	DHN9_F	aagacccgtgggatactgcatcgct	96
Contig1718_s_at	AT3G50980.1	DHN9_R	gtcgccatgtgctgctggttgtc	96
Contig9547_at	AT2G26830.1	CEK4_F	ggcactcattcaggcaagggta	95
Contig9547_at	AT2G26830.1	CEK4_R	ctcctcagtgaagaaaggaagcctt	95
HVSMEf0019H18r2_s_at	AT4G01470.1	TIP1;3_F	atccatgcgtcatcgccatga	96
HVSMEf0019H18r2_s_at	AT4G01470.1	TIP1;3_R	tgactgactcacacacagtttaccc	96
Contig10112_at	AT4G40060.1	HB16_F	gatcctcggacagcgactcgagcg	99
Contig10112_at	AT4G40060.1	HB16_R	tgtccaggaacgacgcgccgaa	99

^aAffymetrix 22 K Barley1 GeneChip Genome Array probe set ID
^bA. thaliana locus identifier corresponding to individual IDs
^cF forward, R reverse, NPH4 Transcriptional factor B3 family protein, PIP plasma membrane intrinsic protein, aquaporin, PI3PK phosphatidylinositol-3P 5-kinase, LTPG5 glycosylphosphatidylinositol-anchored lipid transfer protein 5, PGLAC phospholipid/glycerol acyltransferase, WSI76 galactinol synthase, PRMT10 histone-arginine-N-methyltransferase, ABC ATP-binding cassette transporter, NCED 9-cis-epoxycarotenoid dioxygenase, DHN6 dehydrin DHN6, GLTP glycolipid transfer protein, WRAB1 ABA-inducible protein WRAB1, DHN9 dehydrin DHN9, CEK4 choline/ethanolamine kinase 4, TIP1;3 tonoplast intrinsic protein 1;3, HB16 homeobox protein 16
^dAmplification efficiency

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