Library type | Method description | Multiplex PCR prior to sequencing? | Mean library yield | Index swap rate (%)a | ||
---|---|---|---|---|---|---|
MiSeq | HiSeqX or 4000 | NovaSeqb | ||||
PCR-free genomes | DNA shearing + adapter ligation | - | 2.8 nM | 0.13 ± 0.08 | 3.01 ± 0.91 | 4.85 ± 0.88 |
PCR-plus genomes | DNA shearing + adapter ligation | - | 141.1 nM | 0.03 ± 0.04 | 0.24 ± 0.06 | no data |
Somatic exome | DNA shearing + adapter ligation | + | 354.2 nM | 0.67 ± 0.08 | 0.83 ± 0.23 | 0.52% |
Germline exome | Nextera transposase | + | 286.2 nM | 0.49 ± 0.12 | 0.68 ± 0.19 | no data |
Stranded mRNA | cDNA prep + adapter ligation | - | 39.8 nM | 0.01 ± 0.001 | 0.32 ± 0.02 | no data |