Table 2 Swap probability calculations for Human & E. coli library mixture experiment
| Â | Read count |
|---|---|
Total PF indexed readsa | 842,853,260 |
Total Non-swapped reads | 807,029,454 |
Total swapped reads | 34,219,842 |
p(Total Swap) = (Total swapped reads) / (Total PF indexed reads) = 0.0406 | |
 Undetermined i7 or i5 swaps (Human to Human or E. coli to E. coli swaps) | 17,136,498 |
 Known i7 swaps (Human to E. coli or E. coli to Human swaps) | 5,300,327 |
 Known i5 swaps (Human to E. coli or E. coli to Human swaps) | 12,697,618 |
 Known double i7 and i5 swaps (Human to E. coli or E. coli to Human swaps) | 689,363 |
 Estimated total i7 swaps = i7 / (i5 + i7) * undetermined + i7 + double swaps | 110,36,324 |
 Estimated total i5 swaps = i5 / (i5 + i7) * undetermined + i5 + double swaps | 25,476,845 |
p(i7 Swap) = (Estimated total i7) / (Non-swap + Undet. + Known i5 + Known i7 + Double) = 0.0131 | |
p(i5 Swap) = (Estimated total i5) / (Non-swap + Undet. + Known i5 + Known i7 + Double) = 0.0302 | |