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Fig. 3 | BMC Genomics

Fig. 3

From: Low incidence of SNVs and indels in trio genomes of Cas9-mediated multiplex edited sheep

Fig. 3

Validation of the 2.4 kb inversion at MSTN locus in founder #25. (a) Schematic distribution of SVs in the edited animals. (b) Diagram of the MSTN gene targeted by CRISPR/Cas9 with two sgRNAs. The sgRNA-targeting sequences are highlighted. The inversion region is marked with light blue background. The protospacer adjacent motif (PAM) sequences of NGG are highlighted in red. The positions of the forward and reverse PCR primers used for PCR amplification are indicated by arrows. (c) PCR results confirmed predicted inversion of targeted region at MSTN locus in founder #25

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