Fig. 6

Phylogenetic tree of MAGUK proteins based on their shared MPP PDZ + SH3 + GUK domains. A bayesian tree was inferred with available bilaterian sequences and predicted cnidarian, poriferan, placozoan and ctenophoran sequences aligned with MAFFT v7.123b. MrBayes was run under LG + G model of evolution with 4 rate categories and 1 million generations sampled every 1000 generations. The tree was rooted at midpoint and posterior probabilities are indicated for each branch. The canonical domain architecture was depicted for each main MPP class: MPP5-stardust, CASK, MPP2–6, MPP3–4-7. Whereas Ctenophora lack a MPP5/Sdt orthologue, all 4 sponge classes and Placozoa have one copy of the corresponding gene. Nevertheless, the first Lin2/Lin7 (L27) domain involved in interaction with the L27 domain of PatJ (Pals1-associated tight junction protein) is missing in glass sponges. Domains in grey are predicted but divergent