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Fig. 2 | BMC Genomics

Fig. 2

From: Evaluation of commercially available small RNASeq library preparation kits using low input RNA

Fig. 2

Distribution of total input reads and reads mapped to the transcriptome for liver (L), placenta (P) and brain (B). a Average percentage of input reads for each tissue and kit for the two input amounts (10 ng – top panel; 1 μg – bottom panel) aligned to the human transcriptome (hg19), UniVec contaminants, human rRNA, reads that were too short (< 15nts), and unaligned to the human transcriptome. b Average percentage of reads mapped to the human transcriptome to RNA biotypes for each tissue and kit for the two input amounts (10 ng – top panel; 1 μg – bottom panel): miRNA, tRNA, piRNA, YRNA, snoRNA, snRNA, protein-coding fragments, lincRNA (long intergenic non-coding RNA), antisense RNA, Mt_tRNA (mitochondrial tRNA), MT_rRNA (mitochondrial rRNA), oncRNA (other non coding RNA), miRNA hairpins, reads that are shared between multiple RNA biotypes and reads that are unassigned

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