Table 2 Number of miRNAs differentially expressed in RNA sequencing validated by EdgeSeqa
From: Evaluation of commercially available small RNASeq library preparation kits using low input RNA
Kit | Tissue Comparison | Differentially Expressed miRNAs (RNASeq)a | Assayed by EdgeSeq | Concordant – in same direction as RNASeq (%) | Discordant – in opposite direction as RNASeq (%) | Concordant and Significant (%) |
|---|---|---|---|---|---|---|
BiooScientific | B_vs_L | 322 | 292 (90.68%) | 267 (91.44%) | 25 (8.56%) | 210 (71.92%) |
BiooScientific | B_vs_P | 366 | 305 (83.33%) | 294 (96.39%) | 11 (3.61%) | 243 (79.67%) |
BiooScientific | P_vs_L | 317 | 264 (83.28%) | 252 (95.45%) | 12 (4.55%) | 193 (73.11%) |
Illumina-TruSeq | B_vs_L | 227 | 204 (89.87%) | 197 (96.57%) | 7 (3.43%) | 173 (84.8%) |
Illumina-TruSeq | B_vs_P | 295 | 243 (82.37%) | 235 (96.71%) | 8 (3.29%) | 202 (83.13%) |
Illumina-TruSeq | P_vs_L | 241 | 195 (80.91%) | 184 (94.36%) | 11 (5.64%) | 156 (80%) |
NEB | B_vs_L | 250 | 224 (89.6%) | 218 (97.32%) | 6 (2.68%) | 179 (79.91%) |
NEB | B_vs_P | 312 | 258 (82.69%) | 251 (97.29%) | 7 (2.71%) | 213 (82.56%) |
NEB | P_vs_L | 262 | 213 (81.3%) | 203 (95.31%) | 10 (4.69%) | 168 (78.87%) |